Differential expression of miR-4520a is associated with gain of function mutations in Familial Mediterranean Fever (FMF)
نویسندگان
چکیده
Methods Dual luciferase assay was used to validate a predicted miR-4520a recognition element in the 3’UTR region of the Rheb gene. The expression levels of pyrin, miR4520a and its putative target Rheb were validated in monocytes from FMF patients (n=9) and compared with healthy controls (n=8). Patients were off colchicine for two days (attack-free period) and monocytes were isolated from PBMCs. Total RNA together with the respective miRNA-enriched fractions were isolated from monocytes and used for mRNA and miR-4520a quantitation by real-time PCR using the 2-ΔΔCt method after normalizing to 18S RNA and RNU6B genes, respectively. Protein levels of pyrin and Rheb were detected by western blotting.
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عنوان ژورنال:
دوره 13 شماره
صفحات -
تاریخ انتشار 2015